CRYSTALLINE GLYCEROPHOSPHATE DEHYDROGENASE FROM RABBIT MUSCLE
نویسندگان
چکیده
منابع مشابه
Crystalline glycerophosphate dehydrogenase from rabbit muscle.
Enzymatic activity was determined in an optical test, based on the measurement of reduced DPN at 340 rnp in the Beckman spectrophotometer. The reaction was carried out in phosphate or cysteine buffer at pH 7. Synthetic dihydroxyacetone phosphate (5) with reduced DPN or racemic cr-glycerophosphate with DPN was used as substrate to measure the forward and backward reactions, respectively. The con...
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The reaction catalyzed by this enzyme consists in the oxidation of a -CHO to a -COO. POSH2 group and represents the first oxidative step in the degradation of carbohydrate in the tissues. Diphosphopyridine nucleotide (DPN) and inorganic phosphate are necessary for this reversible enzymatic reaction. The equilibrium has been investigated by Warburg and Christian (l), Drabkin and Meyerhof (2), an...
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THE reduction of methylene blue by lactic acid, or in other words, the dehydrogenation of lactic acid in the presence of extracts from animal tissues, is now known to be catalysed by a system composed of two enzymes (lactic dehydrogenase and diaphorase flavoprotein) and cozymase [Dewan & Green, 1938; Euler & Hellstrom, 1938]. The chemical reactions involved in this process may be written [Corra...
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A number of reports have appeared in recent years on the mechanism, i.e. the mode of enzyme and substrate interaction, of anaerobic dehydrogenases. For example, the mechanisms of heart muscle lactate dehydrogenase (1)) yeast alcohol dehydrogenase (2), and ribitol dehydrogenase (3) appear to involve a compulsory sequence of enzyme and substrate interaction leading to one or more kinetically impo...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1949
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)56670-9